Types for 1 849 patients with urinary calculi and patients' clinical characteristics / 中南大学学报(医学版)

To analyze types of urinary calculi and patients' clinical characteristics, and to explore the strategies for prevention and treatment of urinary calculi.
 Methods: A total of 1 849 patients with urinary calculi were treated in the Department of Urology, the Third Xiangya Hospital of Central South University. The components were analyzed by infrared spectroscopy. The relationship between stone composition and clinical parameters was analyzed according to the clinical characteristics of the patients.
 Results: The proportion of calcium oxalate stone or uric acid stone in male (84.1% or 7.7%) was higher than that in female (78.4% or 4.2%). The older patients were more likely to be diagnosed as uric acid stone. The proportions of uric acid stone in patients <18 years old, 18-<41 years old, 41-<66 years old, and ≥66 years old were 0.0%, 1.6%, 6.6%, and 12.4%, respectively. There was no significant difference in the proportion of stones in patients with different BMI. There were no significant difference in the stone composition between the patients with or without urinary tract infection, hypertension or diabetes. The proportion of uric acid stones in patients with acidic urine was higher than the other types. The proportion of uric acid stones in patients with elevated creatinine (12.1%) was higher than that in the patients with normal creatinine (4.5%).
 Conclusion: Elderly patients, or patients with high uric acid and renal insufficiency are more prone to uric acid stones. Regulation of urinary pH may be an important strategy for preventing and treating urinary calculi in Hunan Province.

Application of a new composite mineralized collagen membrane in alveolar ridge preservation in dogs / 实用口腔医学杂志

Objective:To study the feasibility of a new composite mineralized collagen membrane in alveolar ridge preservation.Methods:The third mandibular premolars on both sides were extracted from 12 dogs,the 24 alveolar sockets were randomly assigned into A,B and C groups(n =8).The distal alveolar sockets of group A was immediately implanted with the new composite mineralized collagen membrane and bone graft material,those of group B with bone graft material,group C was the blank control.The healing of sockets was evaluated by gross observation,morphological measurements,X-ray microscope and photographs of spiral CT.Results:The horizontal width of the alveolar process of group A was bigger than that of group B (P ＜ 0.05),that of group B was bigger than that of group C (P ＜ 0.05).The region of extraction interest in spiral CT value was higher in group A than that in group B and C (P ＜ 0.05).The extraction sockets were generated new bone and the degree of reconstruction measurements was higher in group A than that in group B and C (P ＜ 0.05).Conclusion:The new composite mineralized collagen membrane can induce the regeneration of new bone,and preserve the alveolar.

Study on bone regeneration with two different ratios of nano-hydroxyapatite and collagen grafts after tooth extraction in dogs / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To compare the effect of bone regeneration with two different ratios of nano-hydroxyapatite(nHA)/collagen(Col) (nHAC) after tooth extraction in canine.</p><p><b>METHODS</b>Two kinds of nHAC grafts were prepared with different nHA/collagen ratio of 3∶7 and 5∶5. Eighteen male healthy adult dogs had been randomly divided into three groups. Immediately after extraction of the mandibular second premolars, each kind of nHAC was implanted into extraction sockets as follow: Group A, nHA/Col=3∶7(12 sites); Group B, nHA/Col=5∶5(12 sites); Group C, blank control group(12 sites). The bone repairing abilities of the two grafts such as vertical distance of alveolar, CT values, general observation, histological observation, trabecular volume fraction and porosity were separately analyzed at 1st, 3rd and 6th month, respectively (each group had 4 sites in different time periods).</p><p><b>RESULTS</b>nHAC were absorbed gradually after they were implanted into alveolar bone defect and were replaced by new bone. The vertical distance of alveolar bone in Group A([15.76±0.28] mm) was significant higher than that in Group B([14.88±0.36] mm), and CT values of Group A([879±31] HU) were higher than those in Group B([718±29] HU) (P<0.05). The trabecular bone volume percentage of Group A([22.2±0.4]%) was higher than that in Group B([20.3±0.4]%), and the bone porosity of Group A([23.6±0.9]%) was lower than that in Group B([27.6±0.6]%) (P<0.05). In addition, the vertical distance, CT values and the trabecular bone volume percentage of Group C was lower than those in Group A and B, but the bone porosity of Group C was higher.</p><p><b>CONCLUSIONS</b>The nHAC with nHA/collagen ratio of 3∶7 could better promote bone regeneration than nHAC with the nHA/collagen ratio of 5∶5 did.</p>

Nano-collagen artificial bone for alveolar ridge preservation in the Kazakh from Xinjiang Tacheng Region / 中国组织工程研究

BACKGROUND:It is an important issue of alveolar ridge preservation after tooth extraction. Because of the limited treatment and geographical conditions, lack of awareness of oral health, many local people in frontier areas in Xinjiang have poor alveolar ridge that is not of timely repair after tooth extraction. Thus, it is important to find a suitable local and efficient way to save the residual ridge, which has a more practical clinical value. OBJECTIVE:To investigate the feasibility of nano-colagen artificial bone used for alveolar ridge preservation in the Kazakh in Xinjiang Tacheng Region, China. METHODS: Sixty-eight Kazakh patients with bilateral extraction from Tacheng region, Xingjiang Uygur Autonomous Region, China were selected in a self-controled trial. According to the principle of a minimum alocation imbalance index, the experimental side and control side of extraction patients were confirmed. In the experimental side, nano-colagen artificial bone was implanted; while, conventional treatment was done in the control side. Multi-slice spiral CT was used to scan the regions of extraction interest to measure the relative gray value of alveolar bone mineral density immediately and 3 months after implantation. RESULTS AND CONCLUSION:Immediately after implantation, tooth extraction sockets were visible on CT images both in the experimental and control sides; but after 3 months, the extraction sockets became unclear on the CT images, and CT values were close to those of the surrounding alveolar process, but bone tissues were ful of the bone graft area in the experimental side. The alveolar bone mineral density was higher in the experimental side than the control side at 3 months after implantation. These findings indicate that the nano-colagen artificial bone has good clinical achievement in alveolar ridge preservation in the Kazakh in Xinjiang Tacheng Region.

The effect of bone morphogenetic protein-2 and osteoprotegerin in trans-sutural distraction osteogenesis / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To determine if locally administered bone morphogenetic protein-2 (BMP-2) and osteoprotegerin (OPG) improved osteogenesis and new bone formation by trans-sutural distraction osteogenesis.</p><p><b>METHODS</b>Twenty four dogs were divided into three groups randomly and received new internal trans-sutural distraction osteogenesis treatment. Five days after operation, infusion apparatus with double-tube was inserted to submucosa near the distracted zone to deliver controlled release agent of recombinant human bone morphogenetic protein-2/poly (lactic-co-glycolic acid)/fibrin sealant (rhBMP-2/PLGA/FS) in group A and group C. Recombinant human osteoprotegerin/fibrin sealant (rhOPG/ FS) was injected three weeks later in group B and group C. Histology staining and bone histomorphometry were used to measure the changes of maxillary bone sutura after distraction for 1, 2, 4 and 6 weeks.</p><p><b>RESULTS</b>New bone formation observed in distracted zone showed a significant increase in group A and C. Transmission electron microscope showed the osteoblast and osteocyte were active with dilated rough endoplasmic reticulum and a large number of chondriosomes and Golgi complex. After distraction for 6 weeks, indexes of osteoblast of group A, B, and C were 38.5 +/- 7.7, 35.7 +/- 6.5, and 41.7 +/- 11.0, indexes of osteoclast (Ioc) were 5.9 +/- 1.0, 1.2 +/- 0.3, and 2.8 +/- 0.4, bone trabecula thicknesses were (38.36 +/- 13.28), (66.20 +/- 9.16), and (51.85 +/- 9.92) microm respectively. Increased bone density and decreased Ioc were found in group B and C.</p><p><b>CONCLUSION</b>The new elastic distractor is effective in inducing new bone formation. BMP-2 and OPG combination acts synergistically, and leads to significant enhancement of bone formation and remodeling.</p>

Co-application of muscle-derived stem cells and FK-506 influences nerve regeneration and recovery following acellular nerve allograft implantation / 中国组织工程研究

BACKGROUND: Muscle-derived stem cells (MDSCs) have been accepted as seeding cells in tissue engineered artificial nerves. Tacrolimus exhibits anti-immunologic rejection and promotes nerve regeneration and recovery. Whether can combine these factors with acellular nerve to form a new bridge that can inhibit immunologic rejection and promote nerve regeneration is uncertain. OBJECTIVE: Using the freeze-thawing combined optimized acellular nerve hypotonic-chemical detergent to prepare acellular nerveallograft scaffold. To explore the effect of co-application of MDSCs and FK-506 on nerve regeneration and function recovery following acellular nerve ailograft implantation.METHODS: The sciatic nerve derived from SD rats was prepared nerve bridge after acallular disposal. Gel containing FK-506 and MDSCs was injected into acellular nerveallograft scaffold with 100 μL microsyringe to repair defects. A total of 32 SD rats were randomly divided into 4 groups, with 8 animals in each group. Agap of 10 mm of left sciatic nerve was removed. And then the defects were repaired by extracted nerve graft containing FK-506+MDSCs, MDSCs and FK-506, respectively. In the control group, only hyaluronic acid gel was injected. Sciatic nerve function index (SFI) and electrophysiological exam were performed at weeks 8 and 12 after operation. Gross observation, neurohistological and ultrastructure were observed at week 12 after operation.RESULTS AND CONCLUSION: Compared with the same time point, the SFI, recovery rate of the motor nerve conduction velocities (MNCV), and myelinated nerve fibers in grafting part and in its distal part in the FK-506+MDSCs group were superior to other groups (P < 0.05). The nerve grafts were in normal size with considerabie blood vessels and slightly connected to peripheral Ussues. Compared to other groups, the regenerated nerve fiber in the FK-506+MDSCs group was more density with well-arranged order. A great quantity of Schwann cells proliferated in grafting. The density end diameter of myelinated fiber in the middle and distal part of the grafting were all greater than that of MDSCs and FK-506 groups, and there were few connective tissues between microfascicles, which was close to normal level. The co-application of MDSCs and FK-506 enhances peripheral nerve regeneration and functional recovery in acelluler nerve allograft graft. Therefore, MDSCs and FK-506 are synergistic factors in peripheral nerve injury repair.

Nano-hydroxyapatite/collagen/poly(L-lactic acid)co-cultured with bone marrow stromal cells for repair of rabbit mandibular defect / 中国组织工程研究

BACKGROUND:Selection of proper bone transplantation materials to substitute autogenous bone for treatment of jaw bone defect in patients has become the focus of current researches.OBJECTIVE:To observe the ability of tissue engineered bone constructed with nano-hydroxyapatite/collagen/poly(L-lactic acid)(nHAC/PLA)co-cultured with bone marrow stromal cells(BMSCs)in treatment of rabbit mandibular defect.and to make a comparison with autogenous bone and simple nHAC/PLA.DESIGN.TIME AND SETTING:A randomized controlled animal experiment was performed at the Animal Laboratory of Jinzhou Central Hospital between March and October 2007.MATERIALS:A total of 40 New Zealand rabbits were randomized to 4 groups.with 10 rabbits per group:nHAC/PLA co-cultured with rabbit BMSCs(BMSCs/nHAC/PLA),autogenous bone,nHAC/PLA,and control.METHODS:A 15 mm×15 mm full-thickness bone defect was created in rabbit mandible body.In the BMSCs/nHAC/PLA group.bone defect was repaired by nHAC/PLA co-cultured with BMSCs for 14 days in vitro;the bone defect in the autogenous bone and nHAC/PLA groups was treated by autogenous iliac bone and nHAC/PLA.respectively;while the control group received no any treatment.MAIN OUTCOME MEASURES:At 1,3,and 6 months after surgery,bone mineral density(BMD)measurement and histological examination were performed to evaluate the repairing effects of bone defect.RESULTS:The BMSCs/nHAC/PLA group exhibited the BMD similar to the autogenous bone group(P＞0.05),but it was significantly higher compared to the nHAC/PLA group and the blank control group(P＜0.01).At 6 months after surgery.newly formed bone tissue grew towards large bulk and bridged defected stumps.and the seaffold materials were hardly seen in the BMSCs/nHAC/PLA and autogenous bone groups;simultaneously.the nHAC/PLA group exhibited increased trabecular bone with many fibrous tissues and incompletely degraded seaffold materials.CONCLUSION:BMSCs/nHAC/PLA seaffold exhibits the effects similar to autogenous bone.but superior to nHAC/PLA seaffold in the treatment of mandibular defects.

The influence of hydroxyapatite coating on sandblasted and acid-etched surface of titanium dental implants on the biologic characteristics of marrow-origin osteoblasts / 实用口腔医学杂志

Objective:To study the effects of hydroxyapatite(HA) coating on sandblasted and acid-etched(SLA) surface of titanium dental implants(HA-SLA-Ti) on the biologic characteristics of marrow-origin osteoblasts(MOOBs).Methods:Coating of HA on the SLA surface of titanium dental implants were formed by ion beam assisted deposition (IBAD) method. MOOBs,cultured in vitro,were seeded onto the surface of HA-SLA-Ti and SLA-Ti respectively, the growth of MOOBs on the two kinds of samples were observed by scaning electron microscope.The proliferation index, alkaline phosphatase(AKP) activity, osteocalcin(BGP) content of MOOBs and mRNA relative expression level of osteopontin(opn) were examined and compared between two groups.Results: MOOBs grew well on the surface of HA-SLA-Ti; the proliferation index, AKP activity and BGP content of MOOBs were higher in HA-SLA-Ti group than those in SLA-Ti group(P

The effect of 5-FU polylactic acid nanospheres on the proliferation and apoptosis of human tongue squamous cell carcinoma Tca83 cells / 实用口腔医学杂志

Objective:To study the effect of 5-FU polylactic acid nanospheres(5-FU-PAN)on the proliferation and apoptosis of the human tongue squamous cell carcinoma Tca83 cells.Methods:Tca83 cells were exposed to 5-FU-PAN or 5-FU at the doses(mmol/L)of 1?10-2,2?10-2 and 4?10-2 for 1,3,5,7,9 and 11 days respectively.The survival rates of the cells were examined by MTT assay,the apoptosis of the cells was observed by acridine orange fluorescent staining and the cell cycle distribution was studied by flow cytometry.Results:Both 5-FU-PAN and 5-FU inhibited the proliferation and promoted the apoptosis of the cells dose and time dependantly.5-FU-PAN showed stronger effects than 5-FU.The two agents blocked the cells at S phase and 5-FU-PAN was more potential than 5-FU.Conclusions:5-FU-PAN is more effective in the growth inhibition and apoptosis promotion of Tca83 cells.